|
IgG isotypes for mice of different haplotypes (H-2b, H-2k, H-2q, H-2d). Estimation of IgG isotypes for different peptide formulations in mice bearing haplotypes H-2b(1A), H-2k(1B), H-2q(1C), H-2d(1D). Levels of IgG1, IgG2a, IgG2b and IgG3 and determined as described in Materials and Methods at fixed antisera dilution (1:100). IgG2a,2b,2c and IgG3 were determined using ELISA in all the three bleeds for all the four peptides in different antigenic formulations using the isotyping kit. The O.D. values represent mean ± SEM of sixteen mice (four for each haplotype) as studied for the bleeds obtained on 27th (I), 42nd(II), and 60th (III) day. |
||||||||||||
| Table 1A (H-2b) |
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|
|
||||||||||||
| Formulation |
Peptide in Alum |
Peptide in Liposomes |
Peptide with MA729 in Liposomes |
Peptide in ISCOMs |
||||||||
|
|
||||||||||||
| Isotype↓ Bleed→ |
I |
II |
III |
I |
II |
III |
I |
II |
III |
I |
II |
III |
|
|
||||||||||||
| V3-gp41 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.58 ± 0.10 |
0.56 ± 0.02 |
0.36 ± 0.05 |
0.18 ± 0.02 |
0.32 ± 0.05 |
0.43 ± 0.10 |
0.32 ± 0.06 |
0.58 ± 0.10 |
0.30 ± 0.12 |
0.32 ± 0.10 |
0.45 ± 0.17 |
0.29 ± 0.10 |
| IgG2a |
0.67 ± 0.16 |
0.76 ± 0.10 |
0.45 ± 0.02 |
0.56 ± 0.07 |
0.65 ± 0.14 |
0.50 ± 0.10 |
0.54 ± 0.12 |
0.78 ± 0.20 |
0.73 ± 0.21 |
0.95 ± 0.30 |
1.24 ± 0.26 |
1.10 ± 0.31 |
| IgG2b |
0.78 ± 0.10 |
0.74 ± 0.25 |
0.67 ± 0.10 |
0.98 ± 0.22 |
1.20 ± 0.42 |
1.49 ± 0.36 |
1.35 ± 0.33 |
1.34 ± 0.21 |
1.50 ± 0.18 |
1.54 ± 0.09 |
1.23 ± 0.39 |
1.43 ± 0.32 |
| IgG3 |
0.43 ± 0.17 |
0.32 ± 0.12 |
0.37 ± 0.04 |
0.54 ± 0.10 |
0.43 ± 0.08 |
0.40 ± 0.05 |
0.60 ± 0.10 |
0.70 ± 0.10 |
0.45 ± 0.06 |
0.60 ± 0.10 |
0.58 ± 0.03 |
0.43 ± 0.05 |
|
|
||||||||||||
| gp41 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.10 ± 0.01 |
0.12 ± 0.01 |
0.10 ± 0.03 |
0.10 ± 0.04 |
0.42 ± 0.05 |
1.16 ± 0.10 |
0.10 ± 0.03 |
0.27 ± 0.02 |
0.30 ± 0.06 |
0.10 ± 0.01 |
0.14 ± 0.02 |
0.10 ± 0.01 |
| IgG2a |
0.13 ± 0.02 |
0.21 ± 0.02 |
0.11 ± 0.01 |
0.10 ± 0.01 |
0.52 ± 0.03 |
0.18 ± 0.02 |
0.10 ± 0.01 |
0.60 ± 0.10 |
0.40 ± 0.12 |
0.10 ± 0.01 |
0.20 ± 0.01 |
0.10 ± 0.02 |
| IgG2b |
0.31 ± 0.01 |
0.32 ± 0.13 |
0.23 ± 0.01 |
1.20 ± 0.23 |
1.29 ± 0.32 |
0.10 ± 0.01 |
1.12 ± 0.32 |
1.34 ± 0.02 |
1.31 ± 0.12 |
0.58 ± 0.10 |
1.46 ± 0.23 |
0.78 ± 0.32 |
| IgG3 |
0.13 ± 0.03 |
0.13 ± 0.02 |
0.14 ± 0.01 |
0.09 ± 0.01 |
0.18 ± 0.01 |
0.21 ± 0.02 |
0.41 ± 0.03 |
0.28 ± 0.01 |
0.58 ± 0.10 |
0.08 ± 0.01 |
0.32 ± 0.03 |
0.2 ± 0.04 |
|
|
||||||||||||
| p17 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.58 ± 0.10 |
0.64 ± 0.24 |
0.65 ± 0.03 |
0.72 ± 0.18 |
0.70 ± 0.17 |
0.84 ± 0.34 |
0.64 ± 0.28 |
0.79 ± 0.03 |
0.63 ± 0.16 |
0.69 ± 0.18 |
0.60 ± 0.10 |
0.70 ± 01.8 |
| IgG2a |
0.85 ± 0.20 |
0.89 ± 0.32 |
0.90 ± 0.09 |
0.80 ± 0.02 |
1.01 ± 0.04 |
0.83 ± 0.34 |
0.85 ± 0.32 |
1.07 ± 0.22 |
0.81 ± 0.31 |
0.70 ± 0.18 |
1.01 ± 0.15 |
0.77 ± 0.24 |
| IgG2b |
0.61 ± 0.21 |
0.79 ± 0.17 |
0.61 ± 0.09 |
0.71 ± 0.14 |
1.16 ± 0.12 |
1.28 ± 0.16 |
0.71 ± 0.09 |
1.18 ± 0.02 |
1.31 ± 0.23 |
0.61 ± 0.03 |
0.87 ± 0.21 |
1.29 ± 0.23 |
| IgG3 |
0.46 ± 0.15 |
0.45 ± 0.14 |
0.35 ± 0.12 |
0.40 ± 0.20 |
0.52 ± 0.21 |
0.59 ± 0.15 |
0.40 ± 0.12 |
0.48 ± 0.08 |
0.51 ± 0.01 |
0.59 ± 0.32 |
0.52 ± 0.10 |
0.50 ± 0.13 |
|
|
||||||||||||
| p24 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.34 ± 0.01 |
0.30 ± 0.08 |
0.35 ± 0.02 |
0.70 ± 0.16 |
0.42 ± 0.12 |
0.53 ± 0.14 |
0.49 ± 0.12 |
0.27 ± 0.03 |
0.47 ± 0.05 |
0.89 ± 0.05 |
0.32 ± 0.04 |
0.48 ± 0.15 |
| IgG2a |
0.59 ± 0.13 |
0.46 ± 0.15 |
0.48 ± 0.14 |
0.73 ± 0.15 |
0.93 ± 0.23 |
0.90 ± 0.32 |
0.76 ± 0.23 |
1.06 ± 0.40 |
0.80 ± 0.20 |
0.60 ± 0.13 |
0.94 ± 0.16 |
0.88 ± 0.15 |
| IgG2b |
0.46 ± 0.17 |
0.72 ± 0.08 |
0.89 ± 0.23 |
0.40 ± 0.02 |
0.90 ± 0.04 |
0.91 ± 0.09 |
0.56 ± 0.17 |
0.90 ± 0.23 |
1.07 ± 0.32 |
0.80 ± 0.10 |
0.96 ± 0.16 |
1.02 ± 0.32 |
| IgG3 |
0.58 ± 0.19 |
0.40 ± 0.08 |
0.42 ± 0.05 |
0.36 ± 0.02 |
0.32 ± 0.05 |
0.35 ± 0.05 |
0.52 ± 0.09 |
0.40 ± 0.13 |
0.35 ± 0.09 |
0.53 ± 0.04 |
0.37 ± 0.10 |
0.34 ± 0.12 |
|
|
||||||||||||
| Table 1B (H-2k) |
||||||||||||
|
|
||||||||||||
| Formulation |
Peptide in Alum |
Peptide in Liposomes |
Peptide with MA729 in Liposomes |
Peptide in ISCOMs |
||||||||
|
|
||||||||||||
| Isotype ↓ Bleed → |
I |
II |
III |
I |
II |
III |
I |
II |
III |
I |
II |
III |
|
|
||||||||||||
| V3-gp41 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.46 ± 0.02 |
0.28 ± 0.03 |
0.42 ± 0.13 |
0.10 ± 0.04 |
0.60 ± 0.10 |
0.63 ± 0.20 |
0.47 ± 0.15 |
0.62 ± 0.12 |
0.38 ± 0.09 |
0.26 ± 0.07 |
0.78 ± 0.12 |
0.67 ± 0.20 |
| IgG2a |
0.91 ± 0.23 |
0.46 ± 0.09 |
0.27 ± 0.07 |
0.93 ± 0.21 |
0.90 ± 0.32 |
0.40 ± 0.09 |
0.50 ± 0.07 |
0.83 ± 0.03 |
0.70 ± 0.04 |
0.54 ± 0.14 |
0.80 ± 0.15 |
0.84 ± 0.16 |
| IgG2b |
0.73 ± 0.18 |
0.62 ± 0.19 |
0.90 ± 0.29 |
1.32 ± 0.30 |
1.24 ± 0.34 |
1.08 ± 0.32 |
0.70 ± 0.10 |
1.07 ± 0.20 |
1.35 ± 0.27 |
0.70 ± 0.17 |
0.80 ± 0.19 |
0.95 ± 0.16 |
| IgG3 |
0.26 ± 0.02 |
0.17 ± 0.03 |
0.61 ± 0.04 |
0.26 ± 0.09 |
0.31 ± 0.10 |
0.29 ± 0.04 |
0.16 ± 0.03 |
0.37 ± 0.04 |
0.18 ± 0.08 |
0.24 ± 0.08 |
0.16 ± 0.02 |
0.26 ± 0.02 |
|
|
||||||||||||
| gp41 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.10 ± 0.03 |
0.11 ± 0.04 |
0.20 ± 0.05 |
0.73 ± 0.16 |
1.04 ± 0.24 |
0.70 ± 0.26 |
0.40 ± 0.06 |
1.27 ± 0.43 |
1.28 ± 0.35 |
0.60 ± 0.02 |
0.30 ± 0.02 |
0.06 ± 0.01 |
| IgG2a |
0.12 ± 0.01 |
0.24 ± 0.10 |
0.20 ± 0.04 |
0.63 ± 0.09 |
1.27 ± 0.26 |
1.02 ± 0.29 |
0.47 ± 0.19 |
1.35 ± 0.09 |
1.41 ± 0.12 |
0.69 ± 0.16 |
0.79 ± 0.11 |
0.60 ± 0.10 |
| IgG2b |
0.25 ± 0.08 |
0.26 ± 0.09 |
0.27 ± 0.07 |
0.94 ± 0.20 |
1.31 ± 0.30 |
1.23 ± 0.19 |
1.32 ± 0.20 |
1.37 ± 0.41 |
1.39 ± 0.32 |
0.97 ± 0.10 |
1.14 ± 0.19 |
1.24 ± 0.16 |
| IgG3 |
0.17 ± 0.20 |
0.07 ± 0.16 |
0.10 ± 0.03 |
0.70 ± 0.09 |
0.47 ± 0.07 |
0.30 ± 0.03 |
0.37 ± 0.03 |
0.47 ± 0.22 |
0.58 ± 0.06 |
0.40 ± 0.03 |
0.31 ± 0.01 |
0.25 ± 0.08 |
|
|
||||||||||||
| p17 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.45 ± 0.10 |
0.53 ± 0.03 |
0.50 ± 0.14 |
0.60 ± 0.17 |
0.67 ± 0.19 |
0.70 ± 0.03 |
0.53 ± 0.12 |
0.60 ± 0.18 |
0.50 ± 0.11 |
0.51 ± 0.10 |
0.61 ± 0.14 |
0.59 ± 0.09 |
| IgG2a |
0.44 ± 0.03 |
0.79 ± 0.13 |
0.60 ± 0.16 |
0.60 ± 0.08 |
0.82 ± 0.23 |
0.68 ± 0.11 |
0.50 ± 0.10 |
0.83 ± 0.07 |
0.67 ± 0.01 |
0.50 ± 0.02 |
0.63 ± 0.17 |
0.60 ± 0.02 |
| IgG2b |
0.38 ± 0.02 |
0.65 ± 0.16 |
0.53 ± 0.03 |
0.48 ± 0.02 |
1.09 ± 0.19 |
1.05 ± 0.18 |
0.45 ± 0.10 |
1.05 ± 0.28 |
1.07 ± 0.21 |
0.46 ± 0.31 |
0.72 ± 0.26 |
1.07 ± 0.20 |
| IgG3 |
0.24 ± 0.09 |
0.32 ± 0.06 |
0.21 ± 0.02 |
0.35 ± 0.08 |
0.43 ± 0.10 |
0.45 ± 0.07 |
0.20 ± 0.01 |
0.36 ± 0.08 |
0.38 ± 0.03 |
0.30 ± 0.02 |
0.33 ± 0.06 |
0.35 ± 0.02 |
|
|
||||||||||||
| p24 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.28 ± 0.03 |
0.38 ± 0.07 |
0.31 ± 0.03 |
0.40 ± 0.10 |
0.59 ± 0.04 |
0.51 ± 0.16 |
0.40 ± 0.18 |
0.50 ± 0.15 |
0.42 ± 0.12 |
0.40 ± 0.12 |
0.60 ± 0.23 |
0.42 ± 0.23 |
| IgG2a |
0.52 ± 0.21 |
0.66 ± 0.22 |
0.69 ± 0.11 |
0.60 ± 0.09 |
0.70 ± 0.09 |
0.62 ± 0.16 |
0.40 ± 0.06 |
0.76 ± 0.03 |
0.78 ± 0.14 |
0.80 ± 0.10 |
0.77 ± 0.21 |
0.48 ± 0.08 |
| IgG2b |
0.51 ± 0.07 |
0.54 ± 0.03 |
0.58 ± 0.09 |
0.70 ± 0.16 |
0.81 ± 0.03 |
0.90 ± 0.31 |
0.70 ± 0.26 |
0.92 ± 0.21 |
0.60 ± 0.22 |
0.80 ± 0.21 |
0.97 ± 0.17 |
0.45 ± 0.05 |
| IgG3 |
0.34 ± 0.10 |
0.32 ± 0.09 |
0.31 ± 0.01 |
0.36 ± 0.10 |
0.88 ± 0.07 |
0.64 ± 0.21 |
0.48 ± 0.01 |
0.52 ± 0.09 |
0.69 ± 0.16 |
0.60 ± 0.20 |
0.72 ± 0.19 |
0.44 ± 0.09 |
|
|
||||||||||||
| Table 1C (H-2q) |
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|
|
||||||||||||
| Formulation |
Peptide in Alum |
Peptide in Liposomes |
Peptide with MA729 in Liposomes |
Peptide in ISCOMs |
||||||||
|
|
||||||||||||
| Isotype↓ Bleed → |
I |
II |
III |
I |
II |
III |
I |
II |
III |
I |
II |
III |
|
|
||||||||||||
| V3-gp41 peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.18 ± 0.07 |
0.14 ± 0.02 |
0.17 ± 0.03 |
0.14 ± 0.01 |
0.10 ± 0.01 |
0.36 ± 0.06 |
0.13 ± 0.02 |
0.32 ± 0.12 |
0.33 ± 0.03 |
0.51 ± 0.02 |
0.63 ± 0.11 |
0.70 ± 0.09 |
| IgG2a |
0.26 ± 0.06 |
0.45 ± 0.05 |
0.56 ± 0.07 |
0.70 ± 0.02 |
0.77 ± 0.01 |
0.89 ± 0.10 |
0.95 ± 0.21 |
0.93 ± 0.12 |
0.90 ± 0.10 |
1.23 ± 0.09 |
1.16 ± 0.02 |
1.31 ± 0.20 |
| IgG2b |
0.64 ± 0.05 |
0.56 ± 0.06 |
0.38 ± 0.07 |
0.90 ± 0.21 |
0.95 ± 0.09 |
1.30 ± 0.16 |
0.94 ± 0.14 |
0.96 ± 0.23 |
1.31 ± 0.33 |
1.24 ± 0.20 |
1.34 ± 0.14 |
1.10 ± 0.27 |
| IgG3 |
0.12 ± 0.02 |
0.10 ± 0.01 |
0.19 ± 0.03 |
0.11 ± 0.01 |
0.17 ± 0.04 |
0.23 ± 0.03 |
0.15 ± 0.06 |
0.26 ± 0.17 |
0.34 ± 0.08 |
0.10 ± 0.02 |
0.20 ± 0.01 |
0.70 ± 0.03 |
|
|
||||||||||||
| gp41 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.24 ± 0.01 |
0.23 ± 0.04 |
0.06 ± 0.01 |
0.41 ± 0.09 |
0.42 ± 0.06 |
0.32 ± 0.02 |
0.34 ± 0.10 |
0.40 ± 0.16 |
0.36 ± 0.10 |
0.30 ± 0.03 |
0.31 ± 0.10 |
0.24 ± 0.05 |
| IgG2a |
0.52 ± 0.04 |
0.57 ± 0.05 |
0.60 ± 0.05 |
0.65 ± 0.04 |
0.77 ± 0.07 |
0.89 ± 0.08 |
0.60 ± 0.10 |
1.28 ± 0.09 |
0.95 ± 0.18 |
0.84 ± 0.21 |
0.73 ± 0.30 |
0.65 ± 0.17 |
| IgG2b |
0.67 ± 0.31 |
0.72 ± 0.08 |
0.25 ± 0.02 |
1.40 ± 0.15 |
1.35 ± 0.19 |
1.01 ± 0.21 |
1.14 ± 0.22 |
1.09 ± 0.34 |
1.46 ± 0.52 |
0.91 ± 0.19 |
0.82 ± 0.21 |
0.72 ± 0.08 |
| IgG3 |
0.18 ± 0.06 |
0.28 ± 0.03 |
0.23 ± 0.02 |
0.51 ± 0.03 |
0.51 ± 0.06 |
0.30 ± 0.01 |
0.28 ± 0.06 |
0.28 ± 0.07 |
0.53 ± 0.17 |
0.29 ± 0.03 |
0.32 ± 0.16 |
0.46 ± 0.20 |
|
|
||||||||||||
| p17 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.34 ± 0.08 |
0.32 ± 0.02 |
0.24 ± 0.17 |
0.76 ± 0.16 |
0.30 ± 0.07 |
0.34 ± 0.01 |
0.38 ± 0.02 |
0.26 ± 0.01 |
0.30 ± 0.06 |
0.39 ± 0.09 |
0.29 ± 0.02 |
0.28 ± 0.01 |
| IgG2a |
0.51 ± 0.01 |
0.36 ± 0.04 |
0.38 ± 0.06 |
0.72 ± 0.02 |
0.90 ± 012 |
0.88 ± 0.21 |
0.74 ± 0.15 |
0.92 ± 0.10 |
0.89 ± 0.19 |
0.56 ± 0.12 |
1.03 ± 0.02 |
0.86 ± 0.20 |
| IgG2b |
0.46 ± 0.02 |
0.71 ± 0.08 |
0.88 ± 0.09 |
0.50 ± 0.23 |
0.95 ± 0.32 |
0.91 ± 0.30 |
0.55 ± 0.23 |
0.92 ± 0.32 |
0.94 ± 0.28 |
0.52 ± 0.09 |
0.99 ± 0.21 |
1.05 ± 0.21 |
| IgG3 |
0.56 ± 0.01 |
0.42 ± 0.14 |
0.40 ± 0.15 |
0.56 ± 0.08 |
0.32 ± 0.02 |
0.36 ± 0.05 |
0.32 ± 0.07 |
0.27 ± 0.08 |
0.35 ± 0.05 |
0.34 ± 0.12 |
0.40 ± 0.11 |
0.34 ± 0.10 |
|
|
||||||||||||
| p24 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.40 ± 0.05 |
0.44 ± 0.08 |
0.38 ± 0.02 |
0.30 ± 0.03 |
0.70 ± 0.10 |
0.60 ± 0.11 |
0.61 ± 0.08 |
0.32 ± 0.03 |
0.42 ± 0.06 |
0.29 ± 0.06 |
0.76 ± 0.13 |
0.50 ± 0.02 |
| IgG2a |
0.54 ± 0.11 |
0.62 ± 0.21 |
0.61 ± 0.06 |
0.76 ± 0.03 |
0.88 ± 0.05 |
1.04 ± 0.18 |
0.67 ± 0.02 |
1.07 ± 0.18 |
1.09 ± 0.21 |
0.63 ± 0.21 |
1.00 ± 0.20 |
1.10 ± 0.17 |
| IgG2b |
0.36 ± 0.08 |
0.32 ± 0.04 |
0.55 ± 0.04 |
0.69 ± 0.14 |
1.08 ± 0.16 |
0.90 ± 0.19 |
0.72 ± 0.07 |
1.18 ± 0.13 |
1.16 ± 0.22 |
1.60 ± 0.07 |
1.19 ± 0.03 |
0.90 ± 0.09 |
| IgG3 |
0.31 ± 0.03 |
0.57 ± 0.07 |
0.38 ± 0.03 |
0.46 ± 0.04 |
0.49 ± 0.09 |
0.50 ± 0.10 |
0.31 ± 0.04 |
0.48 ± 0.05 |
0.42 ± 0.11 |
0.52 ± 0.23 |
0.54 ± 0.02 |
0.32 ± 0.10 |
|
|
||||||||||||
| Table 1D (H-2d) |
||||||||||||
|
|
||||||||||||
| Formulation |
Peptide in Alum |
Peptide in Liposomes |
Peptide with MA729 in Liposomes |
Peptide in ISCOMs |
||||||||
|
|
||||||||||||
| Isotype↓ Bleed → |
I |
II |
III |
I |
II |
III |
I |
II |
III |
I |
II |
III |
|
|
||||||||||||
| V3-gp41 peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.33 ± 0.03 |
0.27 ± 0.02 |
0.42 ± 0.03 |
0.65 ± 0.08 |
0.52 ± 0.02 |
0.47 ± 0.02 |
0.30 ± 0.01 |
0.10 ± 0.06 |
0.10 ± 0.01 |
0.21 ± 0.09 |
0.28 ± 0.02 |
0.45 ± 0.05 |
| IgG2a |
0.49 ± 0.02 |
0.59 ± 0.11 |
0.91 ± 0.19 |
0.69 ± 0.04 |
1.03 ± 0.09 |
1.93 ± 0.19 |
0.46 ± 0.10 |
0.70 ± 0.20 |
0.38 ± 0.04 |
0.54 ± 0.03 |
0.40 ± 0.07 |
0.80 ± 0.01 |
| IgG2b |
0.97 ± 0.03 |
1.81 ± 0.12 |
1.70 ± 0.21 |
1.10 ± 0.21 |
1.74 ± 0.20 |
1.47 ± 0.03 |
1.70 ± 0.32 |
1.32 ± 0.21 |
0.90 ± 0.08 |
1.76 ± 0.03 |
1.24 ± 0.08 |
1.80 ± 0.14 |
| IgG3 |
0.35 ± 0.08 |
0.27 ± 0.04 |
0.51 ± 0.18 |
0.70 ± 0.15 |
0.40 ± 0.08 |
0.65 ± 0.05 |
0.30 ± 0.04 |
0.65 ± 0.02 |
0.32 ± 0.02 |
0.29 ± 0.08 |
0.45 ± 0.03 |
0.42 ± 0.02 |
|
|
||||||||||||
| gp41 Peptide |
||||||||||||
|
|
||||||||||||
| IgG1 |
0.13 ± 0.03 |
0.17 ± 0.02 |
0.12 ± 0.03 |
0.63 ± 0.06 |
0.91 ± 0.05 |
0.43 ± 0.13 |
0.44 ± 0.04 |
0.97 ± 0.23 |
1.20 ± 0.21 |
0.30 ± 0.20 |
0.40 ± 0.01 |
0.52 ± 0.10 |
| IgG2a |
0.23 ± 0.10 |
0.34 ± 0.07 |
0.36 ± 0.09 |
0.87 ± 0.03 |
0.90 ± 0.05 |
1.01 ± 0.09 |
0.54 ± 0.15 |
1.45 ± 0.19 |
1.60 ± 0.28 |
0.76 ± 0.17 |
0.83 ± 0.18 |
0.76 ± 0.13 |
| IgG2b |
0.36 ± 0.12 |
0.45 ± 0.01 |
0.43 ± 0.06 |
1.23 ± 0.02 |
1.21 ± 0.01 |
1.10 ± 0.04 |
1.34 ± 0.09 |
1.43 ± 0.03 |
1.49 ± 0.43 |
1.04 ± 0.09 |
0.97 ± 0.12 |
0.80 ± 0.21 |
| IgG3 |
0.17 ± 0.03 |
0.07 ± 0.01 |
0.10 ± 0.02 |
0.34 ± 0.03 |
0.57 ± 0.08 |
0.74 ± 0.12 |
0.54 ± 0.16 |
0.67 ± 0.09 |
0.60 ± 010 |
0.48 ± 0.02 |
0.67 ± 0.13 |
0.60 ± 0.03 |
|
|
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| p17 Peptide |
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|
|
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| IgG1 |
0.19 ± 0.01 |
0.36 ± 0.05 |
0.28 ± 0.06 |
0.02 ± 0.01 |
0.65 ± 0.21 |
0.50 ± 0.02 |
1.21 ± 0.09 |
0.50 ± 0.10 |
0.67 ± 0.12 |
0.23 ± 0.02 |
0.55 ± 0.19 |
0.81 ± 0.09 |
| IgG2a |
0.27 ± 0.03 |
0.40 ± 0.03 |
0.61 ± 0.12 |
0.95 ± 0.25 |
0.92 ± 0.21 |
0.72 ± 0.01 |
0.85 ± 0.10 |
0.95 ± 0.17 |
0.86 ± 0.18 |
0.77 ± 0.18 |
0.98 ± 0.16 |
0.90 ± 0.32 |
| IgG2b |
0.49 ± 0.07 |
0.68 ± 0.09 |
0.69 ± 0.07 |
0.82 ± 0.06 |
0.85 ± 0.05 |
0.99 ± 0.24 |
0.87 ± 0.14 |
0.95 ± 0.32 |
0.97 ± 0.21 |
0.63 ± 0.01 |
1.05 ± 0.20 |
1.15 ± 0.22 |
| IgG3 |
0.31 ± 0.06 |
0.40 ± 0.04 |
0.40 ± 0.12 |
0.28 ± 0.02 |
0.24 ± 0.02 |
0.23 ± 0.01 |
0.25 ± 0.08 |
0.21 ± 0.01 |
0.21 ± 0.04 |
0.28 ± 0.10 |
0.14 ± 0.01 |
0.22 ± 0.02 |
|
|
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| p24 Peptide |
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|
|
||||||||||||
| IgG1 |
0.24 ± 0.01 |
0.41 ± 0.10 |
0.43 ± 0.18 |
0.20 ± 0.06 |
0.68 ± 0.03 |
0.57 ± 0.08 |
0.24 ± 0.10 |
0.61 ± 0.01 |
0.66 ± 0.14 |
0.22 ± 0.01 |
0.53 ± 0.12 |
0.60 ± 0.12 |
| IgG2a |
0.40 ± 0.07 |
0.53 ± 0.03 |
0.60 ± 0.03 |
0.97 ± 0.07 |
0.99 ± 0.21 |
0.81 ± 016 |
0.95 ± 0.12 |
1.08 ± 0.17 |
0.98 ± 0.07 |
0.90 ± 0.22 |
0.96 ± 0.19 |
0.95 ± 0.09 |
| IgG2b |
0.49 ± 0.02 |
0.67 ± 0.02 |
0.69 ± 0.12 |
0.84 ± 0.21 |
0.85 ± 0.12 |
0.98 ± 0.21 |
0.60 ± 0.08 |
0.93 ± 0.02 |
1.25 ± 0.02 |
0.62 ± 0.11 |
1.05 ± 0.22 |
1.15 ± 0.09 |
| IgG3 |
0.31 ± 0.03 |
0.39 ± 0.12 |
0.40 ± 0.12 |
0.20 ± 01 |
0.31 ± 0.09 |
0.33 ± 0.02 |
0.24 ± 0.01 |
0.25 ± 0.05 |
0.21 ± 0.03 |
0.28 ± 0.02 |
0.24 ± 0.08 |
0.24 ± 0.01 |
|
IgG2a,2b,2c and IgG3 were determined using ELISA in all the three bleeds for all the four peptides in different antigenic formulations using the isotyping kit. The O.D. values represent mean. ± SEM of four mice for the bleeds obtained on 27th (I), 42nd(II), and 60th (III) day. | ||||||||||||
Agrawal et al. Journal of Immune Based Therapies and Vaccines 2003 1:5 doi:10.1186/1476-8518-1-5 |
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