Table 2 |
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|
Measurement of KD value by EIA in high and low responder strains of mice. The binding affinities of the antibodies raised against different peptide formulations as assessed by EIA procedure. The slope of the lines (KD values) was calculated by the regression analysis. |
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| Formulations → Peptide/Strain ↓ |
25 μg peptide in Alum KD (nM) |
5 μg Peptide DRV Alum KD (nM) |
5:50 μg Peptide:MA729 DRV Alum KD (nM) |
5 μg peptide in ISCOMs KD (nM) |
|
|
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| gp41/(BALB/c) |
4.80 ± 0.05 |
1.30 ± 0.02 |
1.40 ± 0.02 |
2.70 ± 0.01 |
| gp41/(CBA/J) |
4.50 ± 0.10 |
1.50 ± 0.05 |
1.10 ± 0.01 |
6.70 ± 0.07 |
| V3-gp41/(BALB/c) |
0.20 ± 0.04 |
0.10 ± 0.01 |
0.05 ± 0.008 |
0.10 ± 0.02 |
| V3-gp41/(CBA/J) |
0.90 ± 0.03 |
0.40 ± 0.04 |
0.70 ± 0.03 |
0.50 ± 0.01 |
| p17 /(FVB/J) |
0.90 ± 0.06 |
0.40 ± 0.05 |
0.20 ± 0.01 |
0.50 ± 0.03 |
| p17 /(BALB/c) |
1.40 ± 0.05 |
0.10 ± 0.02 |
0.20 ± 0.02 |
0.90 ± 0.02 |
| p24 /(BALB/c) |
3.50 ± 0.02 |
0.60 ± 0.04 |
0.50 ± 0.01 |
0.60 ± 0.01 |
| p24 /(CBA/J) |
24.5 ± 0.18 |
5.49 ± 0.03 |
1.00 ± 0.03 |
2.50 ± 0.01 |
|
True affinity of the antibodies was measured for different peptides in second bleed of all the formulations. A fixed dilution of antisera (1:500) was used with various molar concentrations of peptide antigen (10-7 M - 10-10 M). | ||||
Agrawal et al. Journal of Immune Based Therapies and Vaccines 2003 1:5 doi:10.1186/1476-8518-1-5 |
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