Table 3 |
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|
Immuno-reactivity of biotinylated HIV peptides derived from different clades using mice antisera against V3-gp41, gp41 and p17 peptides. Immunoreactivity of biotinylated HIV peptides using mice antisera raised against different HIV peptides. Titer of antisera was defined as the reciprocal of dilution which gave a mean absorbance of 0.5. Run was considered invalid if negative control gave a mean absorbance > 0.5. |
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| Dilution of Antisera |
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|
|
||||||
| Strain/Subtype |
Peptide Sequence |
1:100 |
1:400 |
1:1600 |
1:6400 |
*Titer |
|
|
||||||
| Clade A |
NTRKSV-RIGPGQAF-Y |
3.00 |
1.20 |
0.40 |
0.08 |
1348 |
| Clade B |
NTRKSI-HIGPGRAF-Y |
3.00 |
1.35 |
0.37 |
0.13 |
1318 |
| Clade C |
NTRKSI-RIGPGQTF-Y |
3.00 |
1.27 |
0.28 |
0.09 |
1148 |
| Clade D |
NTRQRT-HIGPGQAL-Y |
2.89 |
1.16 |
0.28 |
0.10 |
1122 |
| Clade E |
NTRTSI-TRGPGQVF-Y |
3.00 |
1.25 |
0.36 |
0.10 |
1258 |
| Clade F |
NTRKSI-HLGPGQAF-Y |
2.90 |
1.07 |
0.32 |
0.13 |
1148 |
| Clade G |
NTRKSI-TIGPGQAF-Y |
2.89 |
1.05 |
0.26 |
0.19 |
1023 |
| MN Bio |
NKRKRI-HIGPGRAF-Y |
2.90 |
0.91 |
0.24 |
0.12 |
933 |
| SF2 Bio |
NTRKSI-YIGPGRAF-H |
2.87 |
1.02 |
0.35 |
0.15 |
1148 |
| BR1 |
NTRKSI-HIGWGRAF-Y |
2.80 |
0.98 |
0.30 |
0.15 |
1047 |
| BR2 |
NTRKSI-HMGW-GRAF-Y |
2.64 |
0.96 |
0.28 |
0.16 |
1023 |
| p17 |
YSVHQRIDIKETKDALEKIDDDNKSKKA |
2.90 |
1.50 |
1.10 |
0.65 |
7000 |
| gp41 |
DRPEGIEEEGERRDRDTS |
2.90 |
2.80 |
1.80 |
1.0 |
7500 |
| No Peptide |
- |
0.12 |
0.05 |
0.06 |
0.04 |
11 |
|
|
||||||
|
* Titer of the antisera is defined as the reciprocal of dilution which gave a mean absorbance of 0.5. Run is considered invalid if negative control gave a mean absorbance ≥ 0.5. |
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|
Agrawal et al. Journal of Immune Based Therapies and Vaccines 2003 1:5 doi:10.1186/1476-8518-1-5 |
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