Table 5 |
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|
Cytokine levels for various peptide formulations by cocktail approach. Cytokine levels as measured for various peptide formulations generated by cocktail approach (The value represents the level of cytokines obtained from wells pulsed with the optimal antigen (peptides alone or in adjuvant systems) concentration which showed maximal T-cell stimulation index for mice primed with cocktail of peptides. |
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| Strain→ |
H-2 Haplotypes |
Cytokines (pg/ml) |
|
|
|
|||
| Formulation↓ |
BALB/c (H-2d) |
CBA/J (H-2k) |
|
|
|
|||
| V3-gp41 Peptide |
623 ± 53 |
504 ± 29 |
IFN-γ |
| 440 ± 19 |
291 ± 38 |
IL-2 |
|
| 41 ± 8 |
35 ± 4 |
IL-4 |
|
| V3-gp41 in Liposomes |
1100 ± 101 |
992 ± 63 |
IFN-γ |
| 487 ± 21 |
321 ± 28 |
IL-2 |
|
| 70 ± 14 |
30 ± 2 |
IL-4 |
|
| V3-gp41 in ISCOMs |
1543 ± 165 |
1312 ± 287 |
IFN-γ |
| 511 ± 28 |
360 ± 14 |
IL-2 |
|
| 60 ± 20 |
42 ± 9 |
IL-4 |
|
| V3-gp41: MA729 in Liposomes |
1210 ± 84 |
1413 ± 152 |
IFN-γ |
| 489 ± 31 |
392 ± 30 |
IL-2 |
|
| 58 ± 2 |
58 ± 6 |
IL-4 |
|
| gp41 Peptide |
589 ± 54 |
623 ± 82 |
IFN-γ |
| 401 ± 16 |
368 ± 41 |
IL-2 |
|
| 62 ± 11 |
48 ± 21 |
IL-4 |
|
| gp41 Peptide in Liposomes |
891 ± 16 |
924 ± 72 |
IFN-γ |
| 519 ± 2 |
400 ± 21 |
IL-2 |
|
| 18 ± 54 |
32 ± 11 |
IL-4 |
|
| gp41 Peptide in ISCOMs |
937 ± 20 |
1321 ± 91 |
IFN-γ |
| 521 ± 9 |
500 ± 48 |
IL-2 |
|
| 61 ± 156 |
51 ± 4 |
IL-4 |
|
| gp41 : MA729 in Liposomes |
1230 ± 19 |
1601 ± 134 |
IFN-γ |
| 620 ± 10 |
635 ± 178 |
IL-2 |
|
| 20 ± 71 |
40 ± 2 |
IL-4 |
|
| p17 Peptide |
453 ± 31 |
368 ± 28 |
IFN-γ |
| 301 ± 31 |
371 ± 18 |
IL-2 |
|
| 16 ± 3 |
20 ± 4 |
IL-4 |
|
| p17 Peptide in Liposomes |
1211 ± 96 |
892 ± 44 |
IFN-γ |
| 520 ± 25 |
421 ± 28 |
IL-2 |
|
| 65 ± 9 |
30 ± 5 |
IL-4 |
|
| p17 Peptide in ISCOMs |
1135 ± 157 |
1031 ± 45 |
IFN-γ |
| 520 ± 25 |
423 ± 21 |
IL-2 |
|
| 45 ± 5 |
31 ± 8 |
IL-4 |
|
| p17 : MA729 in Liposomes |
933 ± 28 |
1214 ± 76 |
IFN-γ |
| 581 ± 34 |
408 ± 16 |
IL-2 |
|
| 53 ± 8 |
55 ± 7 |
IL-4 |
|
| p24 Peptide |
411 ± 40 |
284 ± 33 |
IFN-γ |
| 387 ± 22 |
258 ± 19 |
IL-2 |
|
| 48 ± 7 |
54 ± 9 |
IL-4 |
|
| p24 Peptide in Liposomes |
989 ± 107 |
1123 ± 49 |
IFN-γ |
| 460 ± 42 |
270 ± 29 |
IL-2 |
|
| 55 ± 6 |
34 ± 9 |
IL-4 |
|
| p24 Peptide in ISCOMs |
1007 ± 129 |
1290 ± 121 |
IFN-γ |
| 508 ± 16 |
689 ± 43 |
IL-2 |
|
| 49 ± 8 |
36 ± 2 |
IL-4 |
|
| p24 : MA729 in Liposomes |
1241 ± 68 |
1334 ± 59 |
IFN-γ |
| 500 ± 24 |
621 ± 26 |
IL-2 |
|
| 52 ± 7 |
41 ± 4 |
IL-4 |
|
|
The value represents the level of cytokines obtained from wells pulsed with the optimal antigen concentration (peptides alone or in adjuvant systems) which showed maximal T-cell stimulation index for mice primed with cocktail of peptides. | |||
Agrawal et al. Journal of Immune Based Therapies and Vaccines 2003 1:5 doi:10.1186/1476-8518-1-5 |
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