Figure 4.

Determination of AVP-21D9 IC₅₀ using RAW 264.7 264.7 cell based assay 1.2 nM PA and 0.56 nM LF in a 96 well assay on confluent RAW 264.7 264.7 cells cause 100% cell lysis. The AVP-21D9 was assessed at various concentrations for the ability to inhibit the lethal toxin. From the dose response curve an IC₅₀ values was estimated. AVP-22G12 and AVP-1C6 IC₅₀ determinations were carried out likewise.