Figure 2.

Flow cytometry analysis of tumor-infiltrating cells (I). Leukocytes were isolated from liver parenchyma or metastatic liver tumors as described in the Materials and Methods. After incubation with anti-FcR antibody, cells were stained with pairs of FITC- and PE-labeled antibodies to analyze lymphocyte populations. The following combinations were used; FL1/FL2: αβ TCR/panNK (DX5), CD4/CD8, and CD69/CD8. Average percentages of each quadrant, calculated from three samples, are indicated. Underlined numbers in the CD4/CD8 dot plots show the CD8/CD4 ratio. The histograms show CD69 expression on CD8+ T cells and the percentages of CD69+ cells among CD8+ T cells are indicated. Representative data of three experiments are shown. TIL: tumor-infiltrating leukocyte, HL: hepatic leukocyte.