|
Isotype specific antibodies and streptavidin-PE used in the HPV antibody isotyping assays |
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| Isotype Reagent |
Type or MAb Identity |
Specificity |
Source |
Supplier |
Concentration |
|
|
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| Primary |
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| IgM |
polyclonal |
Monkey IgM (mu chain specific) |
Goat |
Rockland, Gilbertsville, PA |
50 μg/mL |
| IgA |
polyclonal |
Monkey IgA (alpha chain specific) |
Goat |
Rockland, Gilbertsville, PA |
25 μg/mL |
| IgE |
HP-6029 |
Human IgE (epsilon chain specific) |
Mouse |
Southern Biotech, Birmingham, AL |
25 μg/mL |
| IgG1 |
HP-6091or 8c/639 |
Human IgG1 |
Mouse |
Sigma, St. Louis, MO |
10 μg/mL |
| IgG2 |
HP-6014 |
Human IgG2 |
Mouse |
Sigma, St. Louis, MO |
10 μg/mL |
| IgG4 |
HP-6025 |
Human IgG4 |
Mouse |
Sigma, St. Louis, MO |
25 μg/mL |
| Total IgG |
polyclonal |
Monkey IgG (gamma chain specific) |
Goat |
Rockland, Gilbertsville, PA |
5 μg/mL |
| Secondary |
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| PE- Streptavidin |
NA |
NA |
NA |
Rockland, Gilbertsville, PA |
5 μg/mL |
Ruiz et al. Journal of Immune Based Therapies and Vaccines 2005 3:2 doi:10.1186/1476-8518-3-2 |
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