Figure 5.

iDCs loaded with DSP or TMG can prime human lymphocytes to respond to MDA-MB-231 cell extracts. iDCsfrom two healthy female blood donors (Donor A; grey bars. Donor B; black bars), were incubated with the peptides DSP or TMG, or cell extracts from MDA-MB-231 tumour cells (TC) [A] or MCF-12A non-tumour cells (NTC) [B]. The loaded iDCs were incubated [First stimulation (1)] with CD14^- PBMCs from the same individual. The cells were cultured for 10 days and then, incubated for a second time [Second stimulation (2)] with iDCs loaded with either TC [A] or NTC [B]. Supernatants were assayed for IFN-γ (pg/mL) production by ELISA. The label 'DSP(1)/TC(2)' refers to IFN-γ production by CD14^-PBMCs following first and second incubations with iDCs loaded with DSP peptide and TC respectively. Other labels follow a similar paradigm.