Figure 1.

Antibody response in BALB/c mice following immunization with bacterial vaccines prepared in test adjuvants. Control mice were either unimmunized or immunized with vaccines in PBS with no adjuvant. The ΔOD⁴⁹⁰ values were obtained by subtracting absorbance due to normal mouse sera from the experimental ones. Polyclonal antisera obtained from E. coli or S. aureus were evaluated at 1:200 dilutions by ELISA. The results represent the average of three separate experiments (n = 4 mice in each three experiments) ± SEM. A. Anti-E. coli Ab response, B. Anti-S. aureus Ab response. Significant increase of antibody response was observed in the sera of mice after the third immunization (P < 0.05).