Figure 4.

Western blot analyses of antigens recognized in bacterial lysates by mouse anti-sera induced with bacterial vaccine prepared in various adjuvants. Lane 1: Molecular marker; Lane 2: PBS (vaccinated with no adjuvant); Lane 3: IFA; Lane 4: PHIS-01; Lane 5: Phytol. Unimmunized controls or normal mouse sera revealed no protein recognizable by either antiserum. A. Antigens revealed by antisera from the E. coli-vaccinated groups. B. Antigens revealed by antisera from the S. aureus -vaccinated groups.