Table 1

Recovery of E. coli from peritoneal fluid obtained from mice challenged with live E. coli

Bacterial challenge (CFU)
106
107
108

Time
36 hrs post infection
18 hrs post infection
36 hrs post infection
18 hrs post infection
36 hrs post infection

1Control group (non-immunized)
0
2N/A
2N/A
2N/A
2N/A
1PBS
0
3.2 ± 1.1 × 104
4.4 ± 1.2 × 105
2N/A
2N/A
1IFA
0
1.8 ± 0.5 × 104
6.0 ± 1.6 × 104
2N/A
2N/A
1PHIS-01
0
0
0
1.1 ± 0.1 × 104
0
1PHYTOL
0
0
0
2.1 ± 0.5 × 104
0

1 Peritoneal fluid samples from all groups of mice were diluted in LB medium (1:200), and 10 μl of diluted samples were streaked on agar plates using calibrated loops. CFUs were scored 18 and 36 hours following bacterial challenge and recorded as CFU/ml. The results represent the average of two experiments (n = 4 for each group) ± SEM. 2 N/A: Not assessable since all mice were dead within 18 hrs.

Lim et al. Journal of Immune Based Therapies and Vaccines 2006 4:5   doi:10.1186/1476-8518-4-5