Table 1

Monoclonal antibodies used to test the expression of cell surface markers of monocyte-derived macrophages and dendritic cells stimulated or not with CpG-ODN or LPS

MARKER
ANTIBODY
CLONE
SUPPLIER
VALIDATION

CD172a
mouse anti-bovine CD172a
DH59B
VMRD, Pullman, WA
Kydd et al., 1994
CD86
mouse anti-human CD86
2331(FUN-1)
Becton and Dickinson, San Diego, CA
Hammond et al., 1999
MHC I
mouse anti-horse MHC I
CZ3
D. Antczak's laboratory, Cornell University
Lunn et al., 1998
MHC II
mouse anti-horse MHC II
CZ11
D. Antczak's laboratory, Cornell University
Lunn et al., 1998
CD14
mouse anti-human CD14
big10
Biometec, Germany
Steinbach et al., 1998
Negative
mouse anti-canine parvovirus
--
C.Parrish's laboratory, Cornell University
Parrish et al., 1982

Flaminio et al. Journal of Immune Based Therapies and Vaccines 2007 5:1   doi:10.1186/1476-8518-5-1