Table 2

Primer and probe sequences used to measure mRNA expression in monocyte-derived macrophages and dendritic cells

CYTOKINE
PRIMER AND PROBE SEQUENCES
GenBank accession #

IL-12p35
5'-TCA AGC TCT GCA TCC TTC TTC AT-3'
Y11130

5'-CAG ATA GCC CAT CAT CCT GTT G-3'


5'-FAM-CCT TCA GAA TCC GCG CAG TGA CCA-TAMRA-3'




IL-12p40
5'-CAC CTG CAA TAC CCC TGA AGA-3'
Y11129

5'-TGC CAG AGC CTA AGA CCT CAT T-3'


5'-FAM-CAT CAC CTG GAC CTC GGC CCA-TAMRA-3'




IFNα
5'-AGG TGT TTG ACG GCA ACC A-3'
M14540

5'-ACG AGC CGT CTG TGC TGA A-3'


5'-FAM-AGC CTC AAG CCA TCT CCG CGG T-TAMRA-3'




IL-10
5'-GAC ATC AAG GAG CAC GTG AAC TC-3'
U38200

5'-CAG GGC AGA AAT CGA TGA CA-3'


5'-FAM-AGC CTC ACT CGG AGG GTC TTC AGC TT-TAMRA-3'




TNFα
5'-GAT GAC TTG CTC TGA TGC TAA TCC-3'
M64087

5'-TCT GGG CCA GAG GGT TGA T-3'


5'-FAM-TCT CCC CAG CAG TTA CCG AAT GCC TT-TAMRA-3'




TLR9
5'-AAC TGG CTG TTC CTG AAG TCT GTG-3'
DQ157779

5'-TCA ACC TCA AGT GGA ACT GCC C-3'


5'-FAM-AGA GAA CTG TCC TTC AAC ACC AGG-TAMRA-3'




β-actin
5'-TCA CGG AGC GTG GCT ACA-3'
AF035774

5'-CCT TGA TGT CAC GCA CGA TTT-3'


5'-FAM-CAC CAC CAC GGC CGA-TAMRA-3'


Flaminio et al. Journal of Immune Based Therapies and Vaccines 2007 5:1   doi:10.1186/1476-8518-5-1