Table 1

Neutralizing antibody titer (NT titer) (geometric mean) of sera from immunized mice against heterologous H5N1 influenza virus (A/Thailand/1/RPNP/2005). Sera from 5 groups of 5 mice per recombinant protein immunization were collected 1 week after last immunization with 250 μg of recombinant HA or NA, or NS1 or M proteins. Mann-Whitney method

Recombinant proteins

Neutralization titer

ELISA titer

GMT (± SD)

P

GMT (± SD)

P

NA/COS-7

34 (± 8)

0.36

160 (± 78)

0.01

NA/E. coli

13 (± 5)

60 (± 21)

HA/COS-7

91.8 (± 35.7)

0.01

422 (± 175)

0.28

HA/E. coli

22 (± 8)

160 (± 87)

NS1/COS-7

139* (± 35)

0.01

1688* (± 701)

0.01

NS1/E. coli

22 (± 8)

422 (± 175)

M/COS-7

0

NA

40 (± 21)

0.1

M/E. coli

0

22 (± 8)

*Significant (P < 0.05, Mann-Whitney method, NS/COS-7 and other proteins)

Horthongkham et al. Journal of Immune Based Therapies and Vaccines 2007 5:10   doi:10.1186/1476-8518-5-10