Figure 5.

Dose-dependent decrease in CD8-perforin expression as number of phenotypic Treg cells increase. A. Increasing numbers of CD3-treated apoptotic CTCL cells (0.3 × 10⁶, 1.5 × 10⁶, 3.0 × 10⁶) were added to a constant number of DC (10⁵), co-cultured overnight and used to stimulate freshly isolated autologous CTCL cells and CD8⁺ cells. As the percentage of membrane CD3⁺/cytoplasmic CTLA4⁺ cells increased there was an inversely related decrease in the number of membrane CD8⁺/cytoplasmic perforin⁺ cells. B. Increasing numbers of apoptotic CTCL cells (0.10 × 10⁶, 0.5 × 10⁶, 1.0 × 10⁶) were added to 10⁵ DC and co-cultured overnight, followed by addition of 10⁶ purified CTCL cells and CD8⁺ cells. As the percentage of membrane CD4⁺/cytoplasmic FoxP3⁺ cells increased, there was an inversely related decrease in the number of membrane CD8⁺/cytoplasmic perforin⁺ cells. Apoptotic = CD3-treated, Viable = CD4-treated