Table 2

Yield of mature DC from roller bottle cultures and static flask cultures*
Expt #
 Culture system
 PBMC seeded per vessel
 Average floating cells recovered
 Average mature DC (CD83+, CD86hi) recovered
 % yield of mature DC
1**
 850 cm2 roller bottles
 900 × 106
 106 × 106
 85 × 106
 9.4%

T-175 static flasks
 180 × 106
 20 × 106
 19.2 × 106
 10.7%
2**
 850 cm2 roller bottles
 900 × 106
 100 × 106
 82 × 106
 9.1%

T-175 static flasks
 180 × 106
 16 × 106
 10.2 × 106
 5.7%
3***
 850 cm2 roller bottles
 800 × 106
 84 × 106
 23 × 106
 2.9%

T-175 static flasks
 200 × 106
 29 × 106
 6 × 106
 3%

*Human peripheral blood leukopheresis products were seeded and monocytes adhered in the two types of culture vessels, as described in the Methods section. After 4 to 5 days of culture with GM-CSF and IL-4, ITIP cocktail was added to induce DC maturation. On average, 2–3 roller bottles or static flasks were set up for each experiment. The floating cells were harvested one day later and viable cellrecovery was determined by Trypan Blue staining with a hemocytometer followed by FACS staining for CD83 and CD86. The number of mature DC was calculated from the percent CD83+, CD86hi cells using the total number of viable floating cells. The results of three separate experiments are shown.

**Experiment was done directly with normal donor non-G-CSF-mobilized leukopheresis products.

***Experiment was done directly with a G-CSF-mobilized normal donor leukopheresis product, accounting for the lower yield of mature DC.

Campbell-Anson et al. Journal of Immune Based Therapies and Vaccines 2008 6:1   doi:10.1186/1476-8518-6-1