Figure 1.

Recombinant invasive E. coli infects phagocytic and non-phagocytic cells. The macrophage cell line, RAW 264.7 and epithelial cell line, TB1, were incubated with GFP-expressing E.coli (E. coli gfp) or co-expressing invasin (E. coli gfp+inv) at MOI of 10 for 3 hours, washed, and after 24 h in gentimicin media, imaged by fluorescent microscopy. The image shows two representative fields at equivalent scale of each treatment and cell line.